Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice

Authors

  • Anandini Swaminathan Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas
    Lithuania
  • Indrė Libnickienė Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas
    Lithuania
  • Tomas Venckūnas Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas
    Lithuania
  • Hans Degens Department of Life Sciences, Research Centre for Musculoskeletal Science and Sports Medicine, Manchester Metropolitan University, Manchester; Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas
    United Kingdom

DOI:

https://doi.org/10.33607/bjshs.v3i126.1274

Abstract

Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice.

Methods: We assessed differences in glucose tolerance outcomes in 2- (young, n=23) and 23- to 27-month-old (old, n=23) male C57BL/6J mice after an intraperitoneal (IP) or intravenous (IV) injection, or oral gavage (OG) of glucose.

Results: The area under the curve (AUC) of the changes of blood glucose concentration over 2 hours after glucose administration was lower in old than young animals (p<0.001). The AUC was higher after IV than either IP or OG (p<0.001). However, normalized to peak glucose concentration, the time course was similar in young and old animals, and was – except for an earlier peak in IV than OG (p=0.013) – independent of route of administration.

Conclusion: We suggest that 1) to determine glucose tolerance the time course of changes in glucose concentration rather than the AUC, which is significantly affected by excursion magnitude, is preferable; 2) glucose tolerance can be measured equally well with IP, IV and OG; 3) there is no significant age-related difference in glucose tolerance in mice.

Keywords: glucose tolerance test, glucose uptake, aging, glucose intolerance, mice, diabetes.

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Published

2022-11-22

How to Cite

Swaminathan, A. ., Libnickienė, I. ., Venckūnas, T., & Degens, H. . (2022). Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice. Baltic Journal of Sport and Health Sciences, 3(126), 16-21. https://doi.org/10.33607/bjshs.v3i126.1274

Issue

Section

Health, Rehabilitation and Adapted Physical Activity